User:Karmella Haynes/Notebook/BioBrick cloning/2011/01/18

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01/18/11

  • ✓ Cultures (6 hours) & minipreps: KAH175-178 (2 each); 3 mL ea.



Minipreps
> Check with E/P/ScaI digests

Reagent Volume Expected:
1,2. KAH175 = 2221
3,4. KAH176 = 3191
5,6. KAH177 = 3384
7,8. KAH178 = 3395
V0120 vector = 1785, 1440
1/18/11 E/P digests
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
ScaI 1.0
dH2O 8.5
  15 μL --> 37°C/ ~15 min.




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