User:Karmella Haynes/Notebook/BioBrick cloning/2014/12/23

Karmella's BioBrick Cloning

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12/23/14 Planning: Gal4 fusion cloning Planning: Gal4 fusion cloning "Backbone" = Gal4-mCherry-VP64 construct KAH60/pcVN Approach: swap-in activator domain to replace VP64 PCR-amplify backbone (omitting VP64) PCR-amplify insert Plan A: use LCR to ligate insert into backbone Order Primers (01/02/15) Attempt 1: swap-in ATF activation domain; add 5' phosphates to all oligos KAH60 "backbone" (without VP64, includes scars) = 6637 KAH60 F1 KAH60 R1 ATF2 insert = 906 ATF2_f1 ATF2_r1 LCR Bridging oligos LCRb_KAH60_ATF2_1 LCRb_KAH60_ATF2_2