02/14/14
- Miniprep digests: CMV/V0120, MV9
Miniprep Digests
- CMV culture 1 - check with E/P
- CMV culture 2 - check with E/P
- MV9 culture 1 - check with P/X
- MV9 culture 2 - check with P/X
- Used the Zymo miniprep kit
- Pelleted each 5 mL culture at 2000 rpm/ 5 min in old centrifuge
- Resuspended each pellet in 600 μL dH2O before miniprep
Reagent
|
Volume
|
Expected (approx): 1. CMV = 3200, 700 2. CMV = 3200, 700 3. MV9 = 4550, 60 4. MV9 = 4550, 60
|
15 μL/lane; 1% agarose; Ladder
|
DNA(plasmid) |
3.0 μL
|
10X buffer |
1.5
|
PstI |
1.0
|
EcoRI or XbaI |
1.0
|
dH2O |
8.5
|
|
15 μL --> 37°C/ ~10 min.
|
- Gave these to Cameron Gardner to build CMV/MV9
|