User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/08/30

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Biomaterials Design Lab Main project page Next entry Objective To showcase the biomineralization of gold nanoparticles in Bovine Serum Albumin (BSA) so that gold nanoparticles can be created and used for future experiments Protocol 0.0015 mM of BSA (Bovine Serum Albumen) and 0.25mM of HAuCl4(Chloroauric Acid) were mixed along with a 10mL buffer of 50mM acetate buffer (pH 5.46). Note: water was used to remove each compound from its respective weighing boats to the final test tube. The mixture was then transferred to a cuvette and analyzed using a UV Vis (Spectrum 200-800). The mixture was then put into an oven at about 70 degrees Celsius and then analyzed using the UV Vis every half hour. Calculations The measured weight of HAuCl4 needed was determined using the calculations: 0.25 mmol/L HAuCl4 × (1 mol/1000 mmol) = 2.5×10-4 mol/L HAuCl4 10 mL solution × (1 L/1000mL) × (2.5×10-4 mol HAuCl4/1 L) × (196.967 g Au3+/1 mol) = 4.9×10-4 g AuCl4 The measured weight of BSA needed was determined using the calculations: 0.0015 mmol/L BSA × (1 mol/1000 mmol) = 1.5×10-6 mol/L BSA 10 mL solution × (1 L/1000 mL) × (1.5×10-6 mol BSA/1 L) × (66776 g BSA/1 mol) = .0010 g BSA Data The actual measurements of each of the compounds were as follows: BSA: 0.0011g HAuCl4: 0.0004g http://i.imgur.com/8JTSp.jpg http://i.imgur.com/QCnJf.jpg Notes This experiment was done as a group under the leadership of Dr. Hartings at American University.