- To showcase the biomineralization of gold nanoparticles in Bovine Serum Albumin (BSA) so that gold nanoparticles can be created and used for future experiments
- 0.0015 mM of BSA (Bovine Serum Albumen) and 0.25mM of HAuCl4(Chloroauric Acid) were mixed along with a 10mL buffer of 50mM acetate buffer (pH 5.46).
Note: water was used to remove each compound from its respective weighing boats to the final test tube.
- The mixture was then transferred to a cuvette and analyzed using a UV Vis (Spectrum 200-800).
- The mixture was then put into an oven at about 70 degrees Celsius and then analyzed using the UV Vis every half hour.
- The measured weight of HAuCl4 needed was determined using the calculations:
- 0.25 mmol/L HAuCl4 × (1 mol/1000 mmol) = 2.5×10-4 mol/L HAuCl4
- 10 mL solution × (1 L/1000mL) × (2.5×10-4 mol HAuCl4/1 L) × (196.967 g Au3+/1 mol) = 4.9×10-4 g AuCl4
- The measured weight of BSA needed was determined using the calculations:
- 0.0015 mmol/L BSA × (1 mol/1000 mmol) = 1.5×10-6 mol/L BSA
- 10 mL solution × (1 L/1000 mL) × (1.5×10-6 mol BSA/1 L) × (66776 g BSA/1 mol) = .0010 g BSA
- The actual measurements of each of the compounds were as follows:
- BSA: 0.0011g
- HAuCl4: 0.0004g
This experiment was done as a group under the leadership of Dr. Hartings at American University.