User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/09/07

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Objective

  • To showcase the biomineralization of gold nanoparticles in Bovine Serum Albumin (BSA) so that gold nanoparticles can be created and used for future experiments

Protocol

  • 1 mL of 0.0015 mM BSA (Bovine Serum Albumen) and 1 mL of 0.25mM of HAuCl4(Chloroauric Acid) were mixed along with a 8mL buffer of 50mM acetate (pH 5.46).
  • 1 mL of 0.0015 mM BSA (Bovine Serum Albumen) and 1 mL of 0.25mM of HAuCl4(Chloroauric Acid) were mixed along with a 8mL buffer of distilled water (pH 7).
  • Each mixture was then transferred to an individual cuvette and analyzed using a UV Vis (Spectrum 200-800).
  • Each buffer was also analyzed using a UV Vis (Spectrum 200-800)
  • Each mixture was then put into an oven at about 72 degrees Celsius and then analyzed using the UV Vis every half hour.

Calculations

Data

Image:A1.jpg‎ Image:A2.jpg‎ Image:A3.jpg‎

Image:W1.jpg‎ Image:W2.jpg‎ Image:W3.jpg‎

Notes

Purple fibers were visible in the solution. The rising slope indicates that there are AUNPs present in the material. Purple fibers were used at a later date for analysis.


This experiment was done as a group under the leadership of Dr. Hartings at American University.



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