User:Mackenzie K. Kelley/Notebook/Experimental Biochemistry/2017/09/26

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Objective Run a Bradford Assay to measure protein concentration of BSA Description prepared 50 mL of stock solution of BSA, 5 mg in 5 mL of saline recorded UV-VIS spectra between 200 nm and 800 nm Made 6 standard solutions (1 mL each) between 1 μg/mL and 20 μg/mL included stock solution, 200 μL of the Bio-Rad Protein Assay reagent, and Tris/NaCl buffer in microcentrifuge tubes tubes were vortexed and left to sit for 5 mins UV-vis spectrum measurements (between 400-800nm) were obtained for solutions within hour of their preparation duplicate blanks solutions (1ml of Tris/NaCl buffer, 200 μL Bradford reagent, 800 μL buffer) were made and UV-vis spectrum measured Results