User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/17

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June 17, 2013

Absorbance Data

AuNP Dilutions

Image:Abs_data_AuNP_dilutions_0613_REDO.png
The absorbance of the AuNP dilutions made in PBS on 06/13/2013 but taken on 06/14/2013 were retaken after samples were left over the weekend, refrigerated. Absorbance spectra observed as expected.

Previous absorbance data:
Image:Abs_data_AuNP_dilutions_24hr_0614.png

Image:Abs_data_AuNP_dilutions_immediate_0614_REDO.png
The absorbance of the AuNP dilutions made in PBS on 06/14/2013 and taken immediately after making were retaken after samples were left over the weekend, refrigerated. Absorbance spectra observed as expected, with the exception of the 9.4nM AuNPs, which showed an even more exaggerated peak around 250 than previously observed. Since only one of the two solutions showed this peak, it can most likely be attributed to sample contamination.

Previous absorbance data:
Image:Abs_data_AuNP_dilutions_immediate_0614.png

Consolidation

Image:Abs_data_AuNP_dilutions_0613_and_0614_REDOS.png
An overlay of the most recent AuNP dilutions absorbance spectra. Consistent measurements observed.

Fluorescence Data

DNA and ThT Redo

Image:Fluor_data_DNA_ThT_redo.png
Redo: samples were remade at same concentrations but excited at 440nm for ThT (previously samples were excited at 417nm, according to absorbance measurements rather than data from the literature)

Varied DNA Concentrations with 2.5μM ThT=

Image:Fluor_data_2.5uM_ThT_and_varied_DNA.png
The fluorescence of samples; varying DNA concentrations and constant ThT concentration of 2.5μM.

ZnPPIX Samples Under the Same Conditions as ThT

Image:Fluor_data_ZnPPIX_samples_as_of_2013_0617.png
The fluorescence of ZnPPIX samples done at the same concentrations as ThT on 06/03/2013


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