User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/17

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Absorbance Data, AuNP/DNA Attachment Main project page
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July 17, 2013

Absorbance Data: AuNP@SiO2 Dilutions

All samples vortexed before running UV-vis measurements to ensure dispersion of nanoparticles in solution.
Image:2013_0717_5uL_TES-MHA_1.PNG Image:2013_0717_5uL_TES-MHA_2.PNG

Image:2013_0717_10uL_TES-MHA.PNGImage:2013_0717_15uL_TES-MHA.PNG

Image:2013_0717_20uL_TES-MHA.PNG Image:2013_0717_dilution_comparison.PNG

DNA and AuNP Attachment Procedure

Replicate procedure from 07/15/2013 with 268mM sodium citrate buffer.

Absorbance Data

Image:2013_0717_AuNP-DNA_&_sups.PNG
Corrected, with HEPES buffer used as reference solution.
Calculations regarding the percentages of AuNPs and DNA in the solution and each supernatant will be done.

For NIST Tomorrow



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