User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2011/08/31

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Objective

Making buffers for class

Description

  1. Add experimental record here. Include what, how, and why...

Data

Buffers

Stock solutions: 1.8M Acetic Acid

Media: Sodium Acetate Tris-Aquo

Media: Tris Base

Moles of each calculated using the buffer calculator for buffers made and used at 25C

  • Buffer 1

Water

  • Buffer 2

100 mL of 50mM Acetate Buffer pH 3.76

Calculations

Amount of acid component

M=nV

(1.8M)=(0.0045mol)(V)

V=2.5mL

Amount base component

m=MM*n

m=136.08g/mol*0.00048mol

m=0.0544g

    • Actual measurement = 0.0563g
    • Actual pH=3.6
  • Buffer 3

100 mL of 50mM Acetate Buffer pH 4.5

Calculations

Amount of acid component

M=nV

(1.8M)=(0.00307mol)(V)

V=1.67mL

Amount base component

m=MM*n

m=136.08g/mol*0.00192mol

m=0.259g

    • Actual measurement = 0.276g
    • Actual pH=4.3
  • Buffer 4

100 mL of 50mM Acetate Buffer pH 5.5

Calculations

Amount of acid component

M=nV

(1.8M)=(0.00055mol)(V)

V=0.36mL

Amount base component

m=MM*n

m=136.08g/mol*0.0043mol

m=0..591g

    • Actual measurement = 0.6143g
    • Actual pH=5.46
  • Buffer 5

100 mL of 50mM Tris Buffer pH 7.5

Calculations

Amount of Tris

m=0.607g

    • Actual mass m=0.6109g

Titrate to pH 7.5 with 1.8M acetic acid

    • Actual pH=7.55

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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