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Objective
The students will make measurement samples from the stock of malachite green that I made yesterday. We'll measure UV-Vis and fluorescence.
Description
- Add experimental record here. Include what, how, and why...
Data
- Rhodamine samples
- Initial Stock
- 7.24 mg of Rhodamine B
- dissolved in methanol and transferred to sample container
- final mass of 3.3205 g
- water added to 14.39510 g
- 502 ppm
- Sample 1
- 23.67 mg of Rhodamine stock
- water added to 15.93720 g
- 746 ppb
- Sample 2
- 10.32 mg of Rhodamine stock
- water added to 15.61280 g
- 332 ppb
- Sample 3
- 8.26 mg of Rhodamine stock
- water added to 14.43720 g
- 287 ppb
- Sample 4
- 4.62 mg of Rhodamine stock
- water added to 14.10721 g
- 164 ppb
- Sample 5
- 2.11 mg of Rhodamine stock
- water added to 15.94823 g
- 66.4 ppb
Fluorescence measurement parameters
- Excitation wavelength: 555 nm
- Emission wavelength start: 565 nm
- Emission wavelength end: 700 nm
- Excitation slit width: 5.0 nm
- Emission slit width: 5.0 nm
- Scan speed: 200 nm/min
Notes
Malachite green was not fluorescent, so I made samples of Rhodamine B for fluorescence measurements. The students measured the UV Vis of the malachite green and rhodamine samples and the fluorescence of the rhodamine samples.
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Student Pages
RG 2018/09/05
[Alex Lutz 2018/09/05]
User:Raquel Lara/Notebook/481-2018/2018/09/05
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Biomaterials Design Lab
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