User:Mbennie/Notebook/Sequencing/8.10.2007

• GCN4 #1 with VF2 (Template: 3ul Miniprep DNA, .3ul primer, 6.7ul water)
  • Somehow only the forward primer managed to get into the vector

NNNNNNNNNNNNNNNNNCCTATANNNATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA
AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGTAAACAGCTGGAAGACAAAGTTGAAGTTTCTTCCTGCAGTCCGG
CAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCG
CTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCAC
AGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACA
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA
CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAG
TCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGG
TGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGGTTTTTTGTTTGC
AAGCAGCANATTACGCGCANAAAAAAGGATCTCAAGANNATCCTTTGATCTTTCTANGGGNCTGACGCTCANTGNNNCNA
AANTNACGTNNNGGANNTTGNCATGAGATNTCANNANGNNNTNNCCTAGANCTTTNANNNAANNNANTTNNNNNANCNNA
NNNNNNTGANTNANTNGNNNNNNGNTNNNNNNNNNNCNNNNCNATAAANNNNNNNGNNNCNNNNNNNNNNNANNNNNGNN
NNNNANNNTNNNNNNNNNNNNANGNNNNNCNNNNNGANNNNNNNNN

• GCN4 #3 with VF2 (Template: 3ul Miniprep DNA, .3ul primer, 6.7ul water)
  • No significant similarity

NNNNNNNNNNNNTANNTATANNATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGA
TGATTTCTGGAATTCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGTGACGTGGTGCGCCCGCGGTCAAACACTT
CGCACCATTCGGCCTGTTTATCCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCG
AAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATC
AGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAAT
CGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCG
CTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGAC
CGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGG
TAACANGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACNGCTACNCTAGAA
GAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAA
ACCACCGCTGGTAGCGGNGGNTTTTTGTTTGCAAGCAGCNNANTACGCGCANAAAAAAANNNTNTCAAGAAGATCCTTTG
ATCTTTTCTACGGNNTCNGACGCTCNNNGNNNNAAAACTCACGTNNNNGNTTTGGNCANNNNATTATNAAAANGANNNTC
NCNAGATCTTTNNNNNAANNNANTTTNNNTNANNNANNNNNNNTNNNNAANNTNNNNNNNNNNNNGNNNNNNNNNCNNNN
AAANNNNNNNNNNNCNNNNNNNNANNNANNNNANNGNNNNNNNNNNNNNANCNGNNNNNNNNNNNNNNNNN

• IgAbc #8 with VF2 (Template: 1ul PCR DNA, .3ul primer, 8.7ul water)
  • Part 1 and Part 3, but missing Part 2

NNNNNNNNNNNNNAATAGGCGTATACACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGANGATTTC
TGGAATTCGCGGCCGCTTCTAGAGGTAGCACGCAATCTATCTTATTGGATACAGGTGCTTCATTAACACGGCACATTGCA
CAAAAATCACGCGCTGATGCCGAAAAAAACAGTGTTTGGATGTCAAACACCGGTTATGGCCGTGATTATGCTTCCGCACA
ATATCGCCGGTTTAGTTCGAAACGCACGCAAACACAAATCGGCATTGACCGCAGCTTGTCCGAAAATATGCAGATAGGCG
GAGTATTGACTTACTCTGACAGTCAGCATACTTTTGATCAGGCGGGCGGCAAAAATACTTTTGTGCAAGCCAACCTTTAT
GGTAAGTATTATTTAAATGATGCTTGGTATGTGGCCGGCGATATTGGTGCGGGCAGCTTGAGAAGCCGGTTACAAACGCA
GCAAAAAGCAAACTTTAACCGAACAAGCATCCAAACCGGCCTTACTTTGGGCAATACGCTGAAAATCAATCAATTCGAGA
TTGTCCCTAGTGCGGGTATCCGTTACAGCCGCCTGTCATCTGCCTGAAGAGCGCTCTTCAGCCGCTTACTTTGCCAATTA
TGGCAAAGGCGGCGTGAATGTGGGCGGTAAATCCTTCGCCTATAAAGCAGATAATCAACAGCAATATTCAGCAGGCGCCG
CGTTACTGTACCGTAATGTTACATTAAACGTAAATGGCAGTATTACAAAAGGAAAACAATTGGNAAAACAAAAATCCNGA
CAAATTAAAATACAGATTCGTTTCTAATAATACTAGTAGCGNCGCTGCAGTCCNGCAAAAAAGGGCAAGGTGTCACCACC
CTGCCCNTTTTCTTNNAACCGAAAGATACTTCGCGTATGCNNNTNNCGCTCACTGACTCGCTGCGCTNGNCGTCGCTGCN
GNNANNNNNCANNNNNNNNNNNNNAAAAANNNN