User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/26

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Objectives Modify Ag:BSA nanofiber synthesis procedure Prepare Au:Myoglobin nanofiber-coated scaffolds for cell culture Ag:BSA Nanofibers: Results Set 1 (All ratios, room temperature) <br.> Set 2 (All ratios, heated to 80C) <br.> Set 3 (All ratios plus 1uL Au, heated to 80C) <br.> Ag:BSA Nanofibers: Sample Prep Au:Myoglobin Nanofibers: Sample Prep for Cell Culture Solutions: Au: 0.011g in 10mL to make 2.793mM solution Myoglobin: 0.011g in 10mL to make 0.0621mM solution TRIS dopamine: 0.038g dopamine hydrochloride and 0.024g TRIS in 20mL to make 10mM solution at pH 8.46 Plain Scaffolds 4 PLA scaffolds printed for each test tube Three test tubes filled with scaffolds and water Autoclaved Brute Force Nanofiber-coated Scaffolds 4 PLA scaffolds printed for each test tube Gold:Myoglobin (100:1) nanofibers synthesized by above method Nanofibers dried onto PLA scaffolds Scaffolds submerged in water Autoclaved Original Method for Nanofiber-coated Scaffolds 4 PLA scaffolds printed for each test tube Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with scaffolds in solution Autoclaved Polydopamine-coated Scaffolds 4 PLA scaffolds printed for each test tube Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours Rinse with deionized water and allow scaffold to dry overnight Scaffolds submerged in water Autoclaved Polydopamine Nanofiber-coated Scaffolds 4 PLA scaffolds printed for each test tube Scaffolds submerged in 10mM pH 8.5 TRIS dopamine hydrochloride solution for 1 hour Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours Rinse with deionized water and allow scaffold to dry overnight Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with polydopamine-coated scaffolds in solution Autoclaved