User:Monica Lovell/Notebook/Biology 210 at AU

Lab 6: February 19, 2014

Embryology and Zebrafish development:

Zebrafish embryos were observed to see how different variable affected their growth and development. In this lab, we added quantum dots to the zebrafish embryos. Twenty zebrafish embryos were put in Deerpark water as the control group. Twenty zebrafish embryos were put in a low concentration of 0.05mg/ml and twenty zebrafish embryos were put in a high concentration of 0.5mg/ml. The embryos were observed for 14 days, however all but two died in the end probably due to a lack of food source. Below is the observations chart:

Different embryological development was compared and analyzed across several species to observe the differences in nature

---

Lab 5: February 12, 2014

Invertebrates - In order to observe and investigate the invertebrate organisms within our transect #3 a Berlese funnel was constructed to collect organisms from a soil and leaf sample collected from our transects. Since it is the winter, the Berlese funnel wakes up the hibernated invertebrates. The Berlese funnels were observe one week after set up.

Worm observation: Before observing the Berlese funnels, the movement of two earthworms were analyzed. The two invertebrate worms moved by compressing their bodies together and then re-lengthening them, which propels them forward.

Invertebrates in Niche #3 Berlese Funnel

Only two invertebrates were identified in our funnel. Both were mites, which are arthropods.

---

Lab 4: February 5, 2014

Plantae and Fungi – Observing Characteristics

Leaf samples were collected from our niche. Plant and Fungi are very important to our niche. Plants provide food and shelter to vertebrates and invertebrates within our habitat. Fungi decompose organic material so that it can be recycled and reused by the environment. Leaf samples were collected in order to identify the plants within our transect. However, because it is winter, most of the plant life was dead. All the leaves collected were dead except from the evergreen holly bush.

First Sample:

Determined Genus – Cornus Serica

Description – Tree and buds

Vascularization – Yes, dicot

Leaves – rounded with buds on branches

Evidence of reproductive parts – Flowers, buds

Location – Transect Center

Second Sample:

Determined Genus – llex opaca

Description – Tree, small

Vascularization – Yes

Leaves – pointed leaves

Evidence of reproductive parts – red berries

Location – Transect Left side

Third Sample:

Determined Genus – Diospyrus

Description – shrub, low to the ground

Vascularization – Yes, dicot

Leaves – round and oval shaped

Evidence of reproductive parts – n/a

Location – Transect Center

Fungi Observation from Agar plates: White/grayish fungi on Agar plate 10-7. Spores with hyphae filaments are in the plate. Dark gray spores are also present. The determination of fungi was made because these spots were needle filament like, not round and smooth like the growing bacteria.

---

Lab 3: January 23, 2014

Hay Infusion were created to culture the organisms within our transect so they may be studied and analyzed.

Hay Infusion observations week 3:

• Smells like cleaning solution or formaldehyde
• Plant particles are all settled at the bottom
• Dark brown and light brown fuzzies throughout solution
• Solution is muddy brown color with a hint of green
• Significant water loss

Identifying bacteria from Hay Infusions: Bacteria were inoculated on agar petri dishes in order to observe bacteria growth. Bacteria were also inoculated on agar and tetracycline plates to observe whether or not some bacteria within the hay infusion is antibiotic resistance. Tetracycline is a common antibiotic administered to prevent or treat a bacterial infection.

Observed bacteria from 100-fold Serial Dilutions Results: There was a greater amount of bacteria in the Agar plates than there was in the Agar + Tetracycline plates. There was no observable colonies in the 10-9 diluted plates in both the Agar and Agar+Tetracycline. The only lawn was in the Agar 10-3 plate.

Bacteria plate observations:

---

January 22 Lab #2

Hay Infusion: Smell: Dirty water Overall water color: brown-green Top layer: Brown film with white specks Middle layer: Film like particles floating Bottom layer: debris from niche

Some organisms feed off the plant matter so samples in the hay infusion were taken near and away from the plant matter.

Two organisms were found in the hay infusion from Niche #3:

1.Amoeba – Observations:

• Contracts
• False feet
• Consumes food
• Has a “head”/directional
• Moves through a slinking motion
• Non-photosnythesizing

Diagram

2. Paramecium multimicronucleatum – Observations:

• Mobile
• No flagellum
• Cilia
• Nucleus
• Non-photosnythesizing

Diagram

---

January 15 Lab #1 Transect #3

Biotic life: tree bush ivy weeds grass

Abiotic life: pebbles concrete light post mulch cigarette butt

Needs more detail. Answer or address questions in protocol in red font and introduce lab, describe aim of lab, techniques undertaken, describe results including pictures, data etc. SK