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Objective
- Repeat experiment performed on September 27th using H2O as buffer.
- Check for the presence of cells that were plated on September 27th.
Description
- Approximately 0.581 ml HAuCl4 (0.25mM), 0.974ml BSA (15uM), and 8.45ml H2O Buffer were pipetted into a test tube.
- The tube was placed in the oven for 2 hours and was not removed.
- Every 30 minutes, a UV-Vis Spectra was obtained using a Shimadzu 2550 spectrometer.
- Following 2 hours, the test tube was removed, wrapped in aluminum foil and was stored at room temperature.
Data
- The graph below is the UV-Vis Spectra of HAuCl4/BSA/H2O obtained in the wavelength range of 200-800nm. This graph was zoomed in at 550nm, which is why the range is from 400nm to 800nm.
- The graph below exhibits the change in absorbance at 550nm over a period of 2 hours
- Observation showed that the solution was purple and contained no fibers.
Notes
1. Conjugated Gold Nanoparticle Synthesis
- According to the two graphs, the absorbance values of the solution increased without fluctuation.
- Observations showed that a clear purple solution formed. No fibers appeared.
- This indicated that conjugated gold nanoparticles formed.
2. The plates contained no cells.
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