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Made new BSA:AuNP based on 8/29 procedure
Made new Arg:AuNP based on 8/30 procedure
Fluorescence:
- Used excitation and emission windows of 8 nm and a scan peed of 50 nm / min. Started emission scan at λex + 10 nm and scanned a range of 200 nm.
- Prepared dilute (1 – 10 μM) solutions of acridine orange and fluorescein.
- Measured fluorescence of your stock solutions using excitation wavelengths of 308 nm, 440 nm, and 490 nm.
- Measured fluorescence of AuNP using each AA and BSA. Used AuNP formed from the same concentration of HAuCl4 at all three excitation wavelengths. Then, measured the fluorescence of the more concentrated AuNP using the Au:AA = 10:1
- Chose the AuNP solutions that had a photoluminescence band around 440 nm
Combined with acridine orange (at a concentration equal to one used in step 2) and excited at 308 nm. Measured the fluorescence up to 600 nm.
- Combined with fluorescein (at a concentration equal to one used in step 2) and excite at 490 nm. Measure the fluorescence up
Figure 1: Arcidine orange solutions UV-VIS data
Figure 2: Arcidine orange and fluorescing with AA solutions
NK
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