User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/27

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HK022

  • Host: Escherichia Coli
  • Action: tyrosine integrase
  • Structure: length:: 357 aa
  • Target sequence:

attB:: has two inverted repeated sequence flanking an overlap region. inverted repeated sequence (core-type). The overlap region shares sequence with attP. Crossing over occurs at the overlap region. overlap-region::AGGTGAA attP has arm-type sites flanking core-type site.

  • Mechanism:

Integrases from lambda and HK022 recognize the same arm-type site. However, the integrases notice the difference in core-type site. IHF is required.


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