User:Pauline Wonnenberg/Notebook/CHEM 471 Fall 2017/2017/09/20

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Objectives Prepare diluted (1-10µM) solutions of acridine orange and fluorescein Measure Fluorescence of stock solutions Measure fluorescence of AuNP using each AA and BSA Description Prepare diluted (1-10µM) solutions of acridine orange and fluorescein Acridine Orange Calculations 10µM=moles/volume (10µM)*(0.05L)=5E-7mol (5E-7mol)*(301.81g/mol)=1.51E04grams of Acridine Orange Fluorescein Calculations 10µM=moles/volume (10µM)*(0.05L)=5E-7mol (5E-7mol)*(332.31g/mol)=1.66E-4grams of Fluorescein Measure Fluorescence of AA stock solutions Using excitation wavelengths of 308nm, 440nm, and 490nm Scan speed of 50nm/min Settings Excitation wavelength + 10nm Dilution Calculations for each stock Data Stock Solution Fluorescence Phenylalanine Fluorescence