User:Richard G Gardner

Contact Info

Richard G Gardner (an artistic interpretation)

• Richard G Gardner
• University of Washington
• Department of Pharmacology
• University of washington
• Box 357280
• Seattle, WA, 98195-7280
• Email me through OpenWetWare

The Gardner lab is located in the Department of Pharmacology at the University of Washington in Seattle, Washington, USA. http://www.gardnerlab.org

Education

• 2000, PhD, University of California, San Diego
• 1992, BA, Cornell University
• 1990, AA, Bucks County Community College

Research interests

1. Protein ubiquitination
2. Chromatin
3. Ribosome Biogenesis

Publications

1. Gardner RG, Nelson ZW, and Gottschling DE. Ubp10/Dot4p regulates the persistence of ubiquitinated histone H2B: distinct roles in telomeric silencing and general chromatin. Mol Cell Biol. 2005 Jul;25(14):6123-39. DOI:10.1128/MCB.25.14.6123-6139.2005 |
2. Gardner RG, Nelson ZW, and Gottschling DE. Degradation-mediated protein quality control in the nucleus. Cell. 2005 Mar 25;120(6):803-15. DOI:10.1016/j.cell.2005.01.016 |
3. Gardner RG, Shearer AG, and Hampton RY. In vivo action of the HRD ubiquitin ligase complex: mechanisms of endoplasmic reticulum quality control and sterol regulation. Mol Cell Biol. 2001 Jul;21(13):4276-91. DOI:10.1128/MCB.21.13.4276-4291.2001 |
4. Gardner RG, Shan H, Matsuda SP, and Hampton RY. An oxysterol-derived positive signal for 3-hydroxy- 3-methylglutaryl-CoA reductase degradation in yeast. J Biol Chem. 2001 Mar 23;276(12):8681-94. DOI:10.1074/jbc.M007888200 |
5. Bays NW, Gardner RG, Seelig LP, Joazeiro CA, and Hampton RY. Hrd1p/Der3p is a membrane-anchored ubiquitin ligase required for ER-associated degradation. Nat Cell Biol. 2001 Jan;3(1):24-9. DOI:10.1038/35050524 |
6. Gardner RG, Swarbrick GM, Bays NW, Cronin SR, Wilhovsky S, Seelig L, Kim C, and Hampton RY. Endoplasmic reticulum degradation requires lumen to cytosol signaling. Transmembrane control of Hrd1p by Hrd3p. J Cell Biol. 2000 Oct 2;151(1):69-82. DOI:10.1083/jcb.151.1.69 |
7. Wilhovsky S, Gardner R, and Hampton R. HRD gene dependence of endoplasmic reticulum-associated degradation. Mol Biol Cell. 2000 May;11(5):1697-708. DOI:10.1091/mbc.11.5.1697 |
8. Gardner RG and Hampton RY. A 'distributed degron' allows regulated entry into the ER degradation pathway. EMBO J. 1999 Nov 1;18(21):5994-6004. DOI:10.1093/emboj/18.21.5994 |
9. Gardner RG and Hampton RY. A highly conserved signal controls degradation of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase in eukaryotes. J Biol Chem. 1999 Oct 29;274(44):31671-8. DOI:10.1074/jbc.274.44.31671 |
10. Gardner R, Cronin S, Leader B, Rine J, and Hampton R. Sequence determinants for regulated degradation of yeast 3-hydroxy-3-methylglutaryl-CoA reductase, an integral endoplasmic reticulum membrane protein. Mol Biol Cell. 1998 Sep;9(9):2611-26. DOI:10.1091/mbc.9.9.2611 |
11. Gardner RG, Wells JE, Fields MW, Wilson DB, and Russell JB. A Prevotella ruminicola B(1)4 operon encoding extracellular polysaccharide hydrolases. Curr Microbiol. 1997 Nov;35(5):274-7. DOI:10.1007/s002849900253 |
12. Hampton RY, Gardner RG, and Rine J. Role of 26S proteasome and HRD genes in the degradation of 3-hydroxy-3-methylglutaryl-CoA reductase, an integral endoplasmic reticulum membrane protein. Mol Biol Cell. 1996 Dec;7(12):2029-44. DOI:10.1091/mbc.7.12.2029 |
13. Gardner RG, Russell JB, Wilson DB, Wang GR, and Shoemaker NB. Use of a modified Bacteroides-Prevotella shuttle vector to transfer a reconstructed beta-1,4-D-endoglucanase gene into Bacteroides uniformis and Prevotella ruminicola B(1)4. Appl Environ Microbiol. 1996 Jan;62(1):196-202. DOI:10.1128/aem.62.1.196-202.1996 |
14. Gardner RG, Wells JE, Russell JB, and Wilson DB. The cellular location of Prevotella ruminicola beta-1,4-D-endoglucanase and its occurrence in other strains of ruminal bacteria. Appl Environ Microbiol. 1995 Sep;61(9):3288-92. DOI:10.1128/aem.61.9.3288-3292.1995 |
15. Gardner RG, Wells JE, Russell JB, and Wilson DB. The effect of carbohydrates on the expression of the Prevotella ruminicola 1,4-beta-D-endoglucanase. FEMS Microbiol Lett. 1995 Jan 15;125(2-3):305-10. DOI:10.1111/j.1574-6968.1995.tb07373.x |

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