User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/11/03

Drosophila melanogaster Genetics

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Spermatogenesis Dissect GFP-Myb for staining with the other Anti-BAM(1:5) Anti-PCNA(1:500). Poor testis for some reason (old flies?) 14 dissected total in two groups of 7 Dissect tdt-Myb for staining (30' at RT) with Oligreen(1:500) (treat with RNAse(1:10) during incubation) Decent testis 9 flies dissected Fixed all testis in 4% Formaldehyde in PBST for 20' Washed 3 x 5' in 1x PBST Block O/N in 2% NGS