User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/10/18

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Ocean Optics pH 8 We calculated the amount of NaOH needed based on pOH. pH 8 = pOH 6 We had to restart the measurements with a new sample around 1 PM (time), as the temperature of the sample holder had dropped. It needs to be at 80 degrees constantly. Sample Preparation BSA Ocean Optics Solution pH 8 [Au]=0.25 mM [BSA]=3.125 uM Vf=3000 uL Stock Concentrations 160920 [BSA]= 63.1 uM [Au]= 2.39 mM [NaOH]= 1 mM Volumes for making the solution: 148.9 uL BSA 255.6 uL Au 2565.4 uL HOH 30 uL HCl Data Analysis The above chart displays the absorbance at 535.15 nm of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time. The chart shows a gradual increase in absorbance over the run time. The above chart shows the maximum wavelength (nm) of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time. The chart displays a plateau of max wavelength ~485 nm until about ~3800 seconds, where it jumps dramatically to about ~550 nm; then leveling off between 530 and 540 nm. The above chart shows the gradual increase in the maximum absorbance of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time.