User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/11/15

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UV Vis of additional samples We Uv-Vis silver solutions that contain only Fructose and no BSA , for Fructose concentrations 0.0625, 0.5 and 1.25 mM, for pH 4 - 12. Sample preparation for UV-VIS Solutions were prepared with 53.1 mM Fructose, 3.45 mM gold, and 34.3 mM silver. All other stock solutions were as stated in the tables. Data Analysis This chart shows the absorbance spectra of 0.5 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs. This chart shows the A(530) for 0.5 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs. This chart shows the absorbance spectra of 0.0625 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs. This chart shows the A(530) for 0.0625 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs. This chart shows the absorbance spectra of 1.25 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs. This chart shows the A(530) for 1.25 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs. This chart shows the UV-Vis absorption spectra for pH 11 samples with varied concentrations of fructose and 0.25mM gold NPs. This chart shows the a(530) for pH 11 samples with varied concentrations of fructose and 0.25mM gold NPs. This image shows the pH 11 samples of Au NPs with varied concentrations of fructose. From left to right: 0.0625, 0.125, 0.25, 0.75, and 1.25 mM Fructose. This image shows the two samples that produced interesting results. On the left is pH 12 Ag with 1.25 mM Fructose. This sample had an interesting peak aruond 270 nm. On the left is Ag 0.0625 mM Fructose pH 11. This sample had a very high absorbance, which is visually confirmed by the intensity of the yellow color. Notes For 0.0625 mM F, one can see that silver nano particles were formed at pH 7 and 8 , because the solutions are slightly yellow. pH 11 is very yellow, and yields a high absorption peak at 420. At lower pH, the absorption peak seems to shift to higher wavelengths (towards 450 nm). For higher fructose (0.5 mM) one can see that we have no nano particles at pH 4, but increasing NPs for pH 5, 6, 7, 8, 9, 10. pH 11 seems less, and shifted to longer wavelengths (approx 400 nm). For F 1.25 mM, we see NPs formed increasingly starting at pH 5. pH 10 looks more cloudy than others. pH 11 is again the max peak. The absolute most nano particles formed seemed to have occurred at pH 11, F 0.0625. The absorption peak is roughly twice as high as 1.25 F at pH 11. The shift in wavelength needs to be as closely examined as the intensity, as we may be able to draw conclusions on NP size and or shape. For gold, one can see that nano particles formed for all F concentrations. However, the solutions were very dark violet , not slight pink as desired. 0.75 mM F shows an absorption curve different in shape: slightly smaller, shifted, and broader than the other solutions.