User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/07/06

PHIX174 Cell Free Expression

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Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP. Transformed 2.5 μL pBEST-PA-UTR1-deGFP-T500 ligation product into JM109. Parallel negative control: -linker ligation product. Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP. Transformed 2.5 μL pBEST-PA-UTRA-deGFP-T500 ligation product into JM109. Parallel negative control: -linker ligation product. Hypothesis 2: Gene L is necessary for phage propagation. Gel electrophoresis results of yesterday's whole plasmid PCR... Negative control: ΦX174 sense and antisense primers: Reaction: ΦX174 template, ΦX174 sense and antisense primers: Positive control: pWhitescript template, pWhitescript sense and antisense primers: