User:Sim Huay Ping/Notebook/CBE/08/150/2008/12/15

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Preparation of mediums and validating results

Prepared Nutrient Agar in 400ml bottles

Prepared Mueller Hinton Broth

Prepared HEPES buffer (Weigh 0.74g of solid KCl, 0.36g of solid glucose, 500ul of HEPES solution in 100ml of DI water)

Prepared Physiological Buffered Saline (PBS)

Prepared Saborand Dextrose Broth

Brought to autoclave


There were no signs of bacteria growth on the kinetics experiment done last week

Changed the PBS used in the kinetics experiment to MHB to minimize death of the bacteria, which could be caused by the waiting time in between the procedures



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