User:Ssutton/Phospholocator/Strains
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| Box | Name | Construct Description | Strain Description | Method of vector propagation | Promoter | Terminator |
| Samantha Box 2: Phospholocator strains | SST416GHI-1040 | Full-length Cdc28-Phosphoplocator | SST1040 (W303a derivative, MATa, can1::HO-CAN1, ho::HO-ADE2, bar1:ura3, ade2, leu2, trp1, his3, CDC28:CDC28-F88A (this is an analog-sensitive Cdc28, inhibitable by 1-NM-PP1), cdc15-2, ura3. We integrated pCTS1-2xmCherry-SV40NLS-ADH1 in at the ura3 locus). | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST429GHI-1040 | Full-length S15A-Cdc28-Phospholocator (first serine mutated to alanine) | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST430GHI-1040 | Full-length S15D-Cdc28-Phospholocator (first serine mutated to aspartic acid) | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST431GHI-1040 | Full-length S33A-Cdc28-Phospholocator (second serine mutated to alanine) | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST432GHI-1040 | Full-length S33D-Cdc28-Phospholocator (second serine mutated to aspartic acid) | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST416tGHI-1040 | Abridged Cdc28-Phosphoplocator | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST429tGHI-1040 | Abridged S15A-Cdc28-Phospholocator (first serine mutated to alanine) | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST430tGHI-1040 | Abridged S15D-Cdc28-Phospholocator (first serine mutated to aspartic acid) | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST431tGHI-1040 | Abridged S33A-Cdc28-Phospholocator (second serine mutated to alanine) | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST432tGHI-1040 | Abridged S33D-Cdc28-Phospholocator (second serine mutated to aspartic acid) | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST445GHI-1040 | Full-length Fus3-Phospholocator | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST445tGHI-1040 | Abridged Fus3-Phospholocator | SST1040 (see above) | Integrated at his3 | Gal1 | Cyc1 |
| Samantha Box 2: Phospholocator strains | SST1040 | No construct | SST1040 (see above) | |||
| Samantha Box 2: Phospholocator strains | SST1050 | No construct | SST1050 (same as SST1040, except with wild-type Cdc28) |
