User:Steven J. Koch/Notebook/Kochlab/2009/04/18/Osmolyte unzipping data 2003/030124 Journal Entries

Data set notes

copied from http://kochlab.org/files/data/Koch_Data/DataSetNotes/030124.txt

1/24/03 5:49 PM
Protocol is normal Popping / BGB protocol.  However, Popping buffer is made from 2x Popping buffer January 2003.  BGB is actually 10 mg / ml ish.
DNA is end-labeled from December 5 2001.  I diluted 1:2 in BGB 10.
BGB made fresh today.  Antidig diluted fresh last week. Beads diluted fresh today.
Today will be Betaine studies.
I will try probably only 1 M betaine 1x popping buffer plus 1:1000 BsoBI
16 amp laser, sum signal at 1.5 V AOD is 243 units.
0158-0159	1M betaine 500 kHz / s; 1:1000 BsoBI added at 4:19 PM
0160	repeating same tether
0161	repeating same tether with reversal
0162-0163	same as 0158-0159; added fresh enzyme before hand
0164	refolding last tether from 0163
0165	back to normal
0166	michelle data
0167-0168	michelle data, 10,000 reference
0169	same tether, michelle trying reversal at 10,000 initial
0170	same tether, michelle trying reversal at 500 initial

0158.ini

(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)
(segment008)
(segment009)

0159.ini

(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)

0160.ini

(segment-specific comments below)
SAME TETHER AS LAST FILE
(segment000)
(segment001) again (same focus)
(segment002)
(segment003)
(segment004)
(segment005)

0161.ini

(segment-specific comments below)
same tether as last file
(segment000)
(segment001)
(segment002)
(segment003)

0162.ini

(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)

0163.ini

(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)
(segment008)

0164.ini

(segment-specific comments below)
(segment000)
(segment001)
(segment002)

0165.ini

(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)
(segment008)

0166.ini

(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)

0167.ini

(segment-specific comments below)
(segment000)
(segment001)
(segment002)
(segment003)
(segment004)
(segment005)
(segment006)
(segment007)
(segment008)
(segment009)

0168.ini

same tether

(segment-specific comments below)
(segment000)

0169.ini

same tether

(segment-specific comments below)
(segment000)
(segment001)

0170.ini

same tether

(segment-specific comments below)
(segment000)
(segment001)