Objective
- Make serial dilutions of the protein combinations (Soy-Silver, Pea-Silver, Casein Silver, Soy-Gold, Whey-Gold, Rice-Silver, Whey-Silver) that successfully produced nanoparticles.
- Prep MH agar plates for future experimentation.
- Perform Disk Diffusion test on bacteria.
Procedure
- MH agar preparation
- Mix MH agar and ~1000mL of deionized water on hot plate with magnetic stirrer.
- Autoclave using liquid setting for ~1.5 hours.
- Pour on sterile petri dishes and leave out to dry overnight.
- Dilutions
- Serial dilutions of 7 protein combinations (Soy-Silver, Pea-Silver, Casein Silver, Soy-Gold, Whey-Gold, Rice-Silver, Whey-Silver) were made along with a serial dilution of ampicillin.
Results
- 43 plates with MH agar were made.
- Disk diffusion procedure is delayed for tomorrow since preparation of agar lasted entire period.
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