User:Wesley J. Houston/Notebook/MAP/2013/01/24

Building a Reporter Gene

Jan. 24, 2013

Followed basic steps found here for plasmid transfection.
Prepped samples: KAH182, KAH201, V0200 and a control.
- Labeled as 1, 2, 3 and 4.
Samples were pipetted into sterile water.
These samples were the placed into a growth medium, Amp DH5α-T.
- The control was simply used to ensure the samples did not become contaminated.
Next, they were placed into an incubator over night.