User:Wilfred J. Poppinga/Notebook/Wilfreds Project/2009/08/24

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Project name Main project page
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Entry title

  • TODO Safety
  • TODO Engineer
  • TODO Watercampus

Miscellaneous

  • Plasmid isolation
    • Nanodrop MBP-ArsR
    • 45.9 ng/μL
    • 260/280: 1.88
    • 260/230: 2.05

PCR

21 μL mastermix*
1 μL forward primer
1 μL reverse primer
1 μL template
1 μL Taq polymerase

*master mix contains:
100 μL Taq NH4
8 μL dNTP's
80 μL MgCl
652 μL MilliQ water

PCR Reaction

  • Hotstart
    • 95 °C, 2 min.
  • 25 cycles
    • 95 °C, 30 sec.
    • 61 °C, 20 sec.
    • 72 °C, 1.5 min.
  • End
    • 72 °C, 10 min.
    • 4 °C, ∞

Comments



Project name Main project page
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Entry title

  • TODO Insert content here...

Miscellaneous

  • Something I finished

Comments

  • [leave a comment!]



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