User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/26
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New cells, cAMP-precipitation, AKAP79 immunoblot of cAMP-precipitation | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||
SummaryThe current cell line running is in passage 28 and therefore a new one will be started from the freezer. To see what AKAPs are seen in the RII overlay of 19May2010 immunoblots will require the same samples to be put to gel again. The ponceau staining appears to show the same results seen 09May2010. Due to the focus of Groningen on AKAP79, so far the only AKAP confirmed in this cell line, the first antibody will be targetted to this AKAP. Materials & MethodsMaterialsMethodPlating cells from -80 °C D9 P23
Putting samples to gel for SDS-PAGE
Dry Western Blot
Notes
Immunoblot
2x new 8% polyacrylamide gels were prepared
cAMP precipitation & Preparations RII overlay
Results
Discussion
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