User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/28
|Immunoblot AKAP79 (upstate)|| Main project page|
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|Sample||Marker||CSE beads||CSE NAC||CSE Lysate Potter||CSE Lysate RIPA||x||HEK293 RIPA lysate||marker||S0 beads||S0 NAC||S0 Lysate Potter||S0 Lysate RIPA||x||x||x|
Splitting cells into 12-wells?
Split new cells
- For weekend
- Other people had some issues with SDS-PAGE today, so this was cancelled and will be continued next week
- AKAP79 immunoblot
There doesn't seem to be a clear band around 79 kDa. However, the bands between the two AKAP79 antibodies seem to be similar. Perhaps there is something that is not really unspecific binding. It might also be that the stripping was not succesful and that the first antibody is still shown. These bands may however appear with each antibody and not just these. AKAP-KL antibody was looked for but not found, this might have been a nice addition, also to check for the latter theory.