User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/01

Summary

• Gel #2 was tried with fresh APS 10% (previous was 1 month old)
  • Still large blur was seen during and after running the gel.
  • When checking pH of the stacking gel a pH of 7.15 was found instead of 6.8
  • This will prevent the stacking function and would explain the blur seen while running
• New stacking buffer was prepared
  • 18.9 g TRIS
  • 150 mL dH₂O
  • pH 6.8
  • added to 250 mL with dH₂O
• Gel #3 was prepared
  • All other gels stored at 4 °C were discarded
  • To prevent more wasting of samples only marker and loading buffer (4x) was used
  • seemed to run owkay after also running buffer was renewed
• Immunotreatment of blot #2 was performed as described yesterday

Materials & Methods

Materials

Method