Entry title
Today we created new nanoparticles with myoglobin and lysozyme in order to determine the optimal concentration for fiber formation.
Notes
Today we are making HRP and myoglobin and pepsin nanoparticles
Table 1. Preparation of Myoglobin-AuNP solutions
Ratio Myoglobin
|
Amount of gold added(mL)
|
Concentration Myoglobin(M)
|
Volume Myoglobin(mL)
|
Volume Water Myoglobin(mL)
|
5 |
0.494264558 |
0.00005 |
2 |
2.505735442
|
10 |
0.494264558 |
0.000025 |
1 |
3.505735442
|
15 |
0.494264558 |
1.66667E-05 |
0.666666667 |
3.839068775
|
20 |
0.494264558 |
0.0000125 |
0.5 |
4.005735442
|
25 |
0.494264558 |
0.00001 |
0.4 |
4.105735442
|
30 |
0.494264558 |
8.33333E-06 |
0.333333333 |
4.172402108
|
35 |
0.494264558 |
7.14286E-06 |
0.285714286 |
4.220021156
|
40 |
0.494264558 |
0.00000625 |
0.25 |
4.255735442
|
45 |
0.494264558 |
5.55556E-06 |
0.222222222 |
4.28351322
|
50 |
0.494264558 |
0.000005 |
0.2 |
4.305735442
|
Table 2. Preparation of all Lysozyme-AuNP solutions
Ratio Lysozyme
|
Amount gold added (mL)
|
Concentration Lysozyme(M)
|
Volume Lysozyme (mL)
|
Volume Water Lysozyme (mL)
|
30 |
0.494264558 |
8.33333E-06 |
1.611148649 |
2.894586793
|
50 |
0.494264558 |
0.000005 |
0.966689189 |
3.539046253
|
70 |
0.494264558 |
3.57143E-06 |
0.690492278 |
3.815243164
|
90 |
0.494264558 |
2.77778E-06 |
0.53704955 |
3.968685892
|
110 |
0.494264558 |
2.27273E-06 |
0.439404177 |
4.066331265
|
130 |
0.494264558 |
1.92308E-06 |
0.371803534 |
4.133931907
|
150 |
0.494264558 |
1.66667E-06 |
0.32222973 |
4.183505712
|
170 |
0.494264558 |
1.47059E-06 |
0.28432035 |
4.221415092
|
190 |
0.494264558 |
1.31579E-06 |
0.254391892 |
4.25134355
|
210 |
0.494264558 |
1.19048E-06 |
0.230164093 |
4.275571349
|
Table 3. Stock Solution concentrations
Concentration of Myoglobin (M)
|
Concentration Gold(M)
|
Concentration Lysozyme (M)
|
0.000125 |
0.00252901 |
2.58615E-05
|
Table 4. Preparation of Lysozyme Stock solution
Molecular Weight(g/mol)
|
14307
|
mass lysozyme(g) |
0.0037
|
volume solution(mL) |
10
|
Concentration Lysozyme (M) |
2.58615E-05
|
Table 5. Preparation of Myoglobin Stock solution
Molecular weight (g/mol)
|
17600
|
mass myoglobin (g) |
0.022
|
volume solution (mL) |
0.01
|
Concentration (M) |
0.000125
|
|