User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/04/15

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April 15th

Goals

  • Test HRP pellet
  • Spin myoglobin and HB to see if it precipitates
  • UV VIS myoglobin and Hb
    • This will determine if the myoglobin or HB breaks apart after spinning



Procedure

  • use centrifuge to spin down proteins
  • use uv-vis

Notes

  • nothing precipitated out after we spun down the myoglobin and the hemoglobin
  • the hemoglobin and myoglobin both showed activity in the solutions



  • from the data we can see that the gamma method appears to be too concentrated to adequately study it

Data

Image:Screen Shot 2014-04-16 at 5.46.16 PM.png

Image:Screen Shot 2014-04-16 at 5.46.24 PM.png

Image:Screen Shot 2014-04-16 at 5.47.09 PM.png

Image:Screen Shot 2014-04-16 at 5.47.02 PM.png

Image:Screen Shot 2014-04-16 at 5.46.34 PM.png

Image:Screen Shot 2014-04-16 at 5.46.45 PM.png

Image:Screen Shot 2014-04-16 at 5.46.53 PM.png


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