WangLab:Inhibitors

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Inhibitors

  1. Jasplakinolide (molecular probes, J-7473): 4 uM 15 min, or 2 M 15 min, 25nM 4h or 50nM 2h give good morphorlogy
  2. ML7: blocking MLCK, 5mM stock, 30 M working concentration.


For HUVECs

  • Cyto D: 0.2 uM, 1 hr
  • Taxol: 0.5 uM, 2 hr
  • Noco: 1 uM, 1 hr


Targeting MoleculeInhibitorsConcentration (uM)Duration (min)
SrcPP210 1 hr
SrcPP1101 hr
SrcSU6656101 hr
RTKSaurumine501 hr
MAPKPD98…101 hr
FAKAG82…201 hr
FAKNVP-TAE2261 uM4 hr?
FLK-1 (VEGFR-2)SU48195 uM1 hr
MicroTubulesColchicine11 hr
MicroTubulesNocodazole11 hr
MicroTubulesPaclitaxel (taxol; stablizing)1 uM2 hr
Actin filamentsCyto D11 hr
Actin filamentsLatrunculin B (sequester G-monomer)0.5 uM1 hr
Actin filamentsJasplakinolite (stabilizing)100 nM1 hr
PI3KWortmannin11 hr
Myosin ATPaseBDM10 mM1 hr
Myosin IIBlebbistatin10 uM1 hr
ROCKY27…101 hr
MLCKML-7; ML-910 or 5; 7.51 hr
PI3KLY 29…501 hr
apoptosisPancaspase inhibitor zVAD-fmk10 uM
de novo protein synthesiscycloheximide25 ug/ml


Targeting MoleculeStimuliConcentration (uM)Duration (min)
RhoALPA200 ng/ml30 min
RacPDGF25 ng/ml30 min
Cdc42bradykinin100 nM3 min
integrins a5b1, avb3, avb5Fibronectin10 ug/ml1 hr
integrins avb3Fibronogen, Vitronectin
integrins a1b1, a2b1collagen
integrins a1b1, a2b1, a3b1 and a6b1laminin


serum starved for 16 hr. Cells were either treated with LPA (200 ng/ml−1) for 30 min (Sigma, Poole, UK), PDGF (25 ng/ml−1) for 30 min (TCS Biologicals, Botolph Claydon, UK), or bradykinin (100 nM) for 3 min (Sigma) or were plated on fibronectin (BD Biosciences) (10 μg/ml) for 1 hr.

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