WangLab:Measure DNA Concentration
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1. Choose DNA protocol -> enter, enter, enter... 2. Choose Dilution Factor
Usually 20x for miniprep product, and 100x for maxprep product.
3. Subtract Background
Blank H2O 200 100 ul (miniprep, 200 ul for maxiprep) -> Read blank
4. Continue -> Read Sample -> Measure twice -> Read sample -> ?
The amount of DNA needed: miniprep: 5 ul dilute 20 times -> 100 ul maxiprep: 2 ul dilute 100 times -> 200 ul ; and measure again after adding water to adjust concentration.