WangLab:Stardard PCR

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Standard PCR

Mg(1.5mM)Mg(2.5mM)Mg(1.5mM)Mg(2.5mM)
1.10x buf (M190G, promega): 5 5 5 5
2.MgCl2 3 5 3 5
3.DNA (miniprep): 1 1 1(1/5) 1(1/5)
4.primer1 (20 uM): 2.5 2.5 2.5 2.5
5.primre 2 (20 uM): 2.5 2.5 2.5 2.5
6.*dNTP: 1 1 1 1
7.*Tag: 0.5 0.5 0.5 0.5
8.H2O: 34.5 32.5 34.5 32.5
  • Run program 10, all are good.
  • Group 4 gives the best results.
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