- Take ice, put DH5a, wait to thaw
- Aliquote 50 ul to prechilled 600 ul tubes
- Add 1 ul DNA to tubes and wait for 30 min on ice.
- Heat shock 42oC for 45 sec.
- Incubate on ice for 2 min
- Add in LB medium 500 ul to each tube
- Shaker in 37oC for 20 min
- 50 ul plate on agar plates