WangLab:Transformation Procedure

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Transformation Procedure

  1. Take ice, put DH5a, wait to thaw
  2. Aliquote 50 ul to prechilled 600 ul tubes
  3. Add 1 ul DNA to tubes and wait for 30 min on ice.
  4. Heat shock 42oC for 45 sec.
  5. Incubate on ice for 2 min
  6. Add in LB medium 500 ul to each tube
  7. Shaker in 37oC for 20 min
  8. 50 ul plate on agar plates
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