Wittrup: Basic Culture Media
The following is a recipe for basic cell culture media; some cell lines will require additional components for growth, so it is best to check the literature.
Basic Culture Media
440 mls media (eg: RPMI, AMEM, DMEM)
50 mls FBS (for 10% by volume)
5 mls L-glutamine
5 mls Pen-Strep (optional antibiotic)
L-glutamine, Pen-Strep, and some medias are available from VWR.