Wittrup: MES running buffer

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Formulation

  • 50mM MES
  • 50mM Tris Base
  • 0.1% SDS
  • 1mM EDTA
  • pH 7.3

1L of 1x Buffer

  • 9.76g MES
  • 6.057g Tris Base
  • 1g SDS
  • 0.292g EDTA
  • pH to 7.3

5L of 1x Buffer

  • 48.8g MES
  • 30.285g Tris Base
  • 5g SDS
  • 1.461g EDTA
  • pH to 7.3
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