Wittrup: MOPS running buffer

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Formulation

  • 50mM MOPS
  • 50mM Tris Base
  • 0.1% SDS
  • 1mM EDTA
  • pH 7.7

1L of 1x Buffer

  • 10.463g MOPS
  • 6.057g Tris Base
  • 1g SDS
  • 0.292g EDTA
  • pH to 7.7

5L of 1x Buffer

  • 52.315g MOPS
  • 30.285g Tris Base
  • 5g SDS
  • 1.461g EDTA
  • pH to 7.7
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