Yu:CEB

Solution recipe for 500mL Calmodulin Elution Buffer.

1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker	25mM Tris-HCl
2. Add 15mL of 5M NaCl	150mM NaCl
3. Add 500 μL of 1M Magnesium Acetate	1mM MgOAc
4. Add 500 μL of 1M Imidazole	1mM Imidazole
5. Add 1 mL 10% NP-40	0.02% NP-40
6. Add 100mL of 100mM EGTA	10mM EGTA
7. Bring volume to 500mL with millipore H2O in a graduated cylinder	Volume to 500mL
8. Complete immediately prior to use with 0.696 μL β-mercaptoethanol per 1 mL CEB	10mM β-mercaptoethanol
9. Keep completed CEB on ice at all times