Yu:TST

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Solution recipe for 1L TST Buffer.
1. Measure 500 mL millipore H2O into a 1L beaker
2. Add 50 mL 1M TRIS-HCl, pH 7.4 50 mM TRIS-HCl, pH 7.4
3. Add 30 mL 5M NaCl 150 mM NaCl
4. Add .5 mL 100% TWEEN-20 0.05% TWEEN-20
5. Bring volume to mL total with millipore H2O in a graduated cylinder Bring volume to 1L
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