Registry/Measurement kit/Notebook/2007-7-4

Colony PCR

• Yet again, yesterday's PCR seems to have evaporated...
  • Trying again with a different tube.

E0240

• Made glycerol stocks of colonies 3,4,6 and mini-prepping the remaining 4mL.
  • Will send for sequencing today.
• Sequencing order: 3F, 3R, 4F, 4R, 6F, 6R

I2057

• Sequence matches!

T9002

• Starting sequential digest (MfeI, then NsiI).

Primers

• Need scarring primers for I2056, I2057
  • I2056:
    • F: Same as I2055
    • R: same as I2055

• • I2057:
    • F: CTTAGTAG CAATTG TCACACAGGAAAGTACTAGATGGC (52.1)
    • R: same as I2055