Smolke:Protocols

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Note: If possible, please follow the standard protocol format. Protocols Cloning Primer Design Plasmid prep with Epoch columns and homemade buffers PCR amplifying inserts from plasmids When to use phenol-chloroform vs. columns Restriction digests Gel extraction Ligation Electrocompetent Transformation Heat Shock Transformation Colony PCR Screening by digestion Sequencing with Elim Making freezer stocks Gene assembly EtBr stock Yeast Protocols Yeast Media Recipes Pouring yeast dropout plates Yeast plasmid transformation Quick Yeast plasmid transformation Yeast colony PCR Anti-V5 Western Library-scale yeast transformations & Gap repair Yeast chromosomal modifications Yeast plasmid prep Feeding yeast cultures Yeast metabolite extraction Heme measurements Observations and Discussion CSY3 Tissue Culture room Adherent cell care and feeding Media stocks Freezer stock management and database General maintenance General sterility Quanta Preventing Clogs Clearing Clogs VYB Guidelines Miscellaneous Glass Beads Poster Printing My pipet-aid is broken! Cleaning Bottles for the LC 96-well plates Solutions Pfu Buffer Taq Buffer 10x DO Media dNTP Mixture 5-Fluoroorotic acid Antibiotic Stocks