User:Anthony Salvagno/Notebook/Research/2011/03/04/Prepping Unzipping With pALS: Difference between revisions
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There is a good chance this doesn't happen today. | There is a good chance this doesn't happen today. | ||
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Revision as of 18:18, 4 March 2011
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PCR
I cleaned up the PCR reaction from Thursday and the nanodrop revealed:
- 227ng/ul --> 85nM in 30ul (total of 6.8ug of DNA)
BstXI Digestion
Error in widget Google Spreadsheet: Unable to load template 'wiki:Google Spreadsheet'
Adapter Annealing
Since I got a new bottom oligo with 5' biotinylation I will have to anneal a new adapter duplex.
Error in widget Google Spreadsheet: Unable to load template 'wiki:Google Spreadsheet'
Ligation
I only have SappBR so I will need to ligate the adapter to here first and then ligate this to the digested pALS.
SappBR-Adapter Ligation
Error in widget Google Spreadsheet: Unable to load template 'wiki:Google Spreadsheet'
ApBR-DpALS Ligation
There is a good chance this doesn't happen today.
