Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.6.08 Master Mix

From OpenWetWare
Revision as of 02:44, 7 August 2008 by Mgethers (talk | contribs) (New page: =8.3.08 Master Mix= {| {{table}} | align="center" style="background:#f0f0f0;"|'''Reagent''' | align="center" style="background:#f0f0f0;"|'''Volume/Amount''' |- |Colony Suspension||16.5 &...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

8.3.08 Master Mix

Reagent Volume/Amount
Colony Suspension 16.5 μl (not added to master, though)
Taq 1.65 μl
Primer BT2736 13.2 μl 1 μM Stock
Primer BT2737 13.2 μl 1 μM Stock
dNTPs 3.3 μl 10 mM Stock
DMSO 8.25 μl
Buffer 16.5 μl
Mg2+ 8.25 μl
dH20 84.15 μl
Total 165 μl

Aliquotted 9 μl of to each of 8 tubes, then added 1 μl appropriate colony suspension.

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Matt_Gethers/CRI,_Thailand/Labwork/PCRs/Amplification_of_HmgR_ORF/8.6.08_Master_Mix&oldid=228937"