Biomod/2012/Titech/Nano-Jugglers/Methods: Difference between revisions
No edit summary |
No edit summary |
||
Line 42: | Line 42: | ||
|- | |- | ||
|width="300px"[[Image: BIOMODPVDM1.png]] | |width="300px"[[Image: BIOMODPVDM1.png]] | ||
|- | |||
| | | | ||
===1st Deposition=== | ===1st Deposition=== | ||
::First, we deposit gold on the polystyrene-bead in order to make gold hemisphered body. | ::First, we deposit gold on the polystyrene-bead in order to make gold hemisphered body. | ||
===2nd Deposition=== | ===2nd Deposition=== | ||
::Next, we change the direction of beads, and deposit chromium on the body. | ::Next, we change the direction of beads, and deposit chromium on the body. Then we pick up polystyrene-bead, which are deposited by quarter gold and half chromium. By chromium capping, this "head part" is prevented to conjyugate DNA strands, so platinum only mounted at the rear. | ||
|} | |||
===After PVD=== | ===After PVD=== | ||
::Through twice of physical vapor deposition, | ::Through twice of physical vapor deposition, | ||
Line 56: | Line 56: | ||
::Therefore, platinum particles can't conjugate onto chromium hemisphere. | ::Therefore, platinum particles can't conjugate onto chromium hemisphere. | ||
::As a result, JET starts to move toward chromium hemisphere heading. | ::As a result, JET starts to move toward chromium hemisphere heading. | ||
| | <div align = "right" style="padding-right:200px">[[#TOP|↑]]</div> | ||
==DNA conjugation== | ==DNA conjugation== | ||
:{| | :{| |
Revision as of 22:33, 30 September 2012
<html> <head> <style type="text/css"> /* ====================
主に全体に関わるCSS
==================== */ body.mediawiki {
font-size: 14px;
background-image:url(http://openwetware.org/images/2/2d/TNJback.png); background-repeat: repeat; font-family: Calibri, Verdana, helvetica, sans-serif; }
h1 { padding: 0px 20px 5px 20px; font-size: 34px; line-height: 40px; font-weight: bold; } h2 { padding: 20px 20px 5px 20px; font-size: 25px; color: #000000; text-decoration: none; font-weight: bold; } h2 a { color: #eb8300; } h3 { padding: 20px 20px 5px 20px; font-size: 20px; color: #000; font-decoration: none; font-weight: bold; } h1.firstHeading {
display: none;
}
p { text-align: justify; } a:link { color: #00a5ea; text-decoration: none } a:visited { color:#00a5ea; text-decoration: none } a:hover {
color: #eb8300;
text-decoration: none } a:active { color:#f29400; text-decoration: none } #content{ margin: 0px 0 0px 0; align: center; padding: 12px 12px 12px 12px; width: 946px; background-color: #ffff; border: 0; } #bodyContent{ width: 970px; margin: 150px auto 0 auto; align: center; background-color: #ffffff; } #globalWrapper{ margin: 20px 0 0 0; width:994px; background-color: #ffffff; margin-left: auto; margin-right: auto } #biomodlink{ position:absolute;top:0px; width:970px;height:20px; background-color: #ffffff; margin:3px auto 3px auto }
/* ====================
メニューの画像を変更できる部分 ==================== */
#header {
margin: 0 auto 0 auto; position:absolute; top:28px; width:968px;height:189px;
background-color: #FFFFFF; background-repeat: no-repeat;
background-image: url(http://openwetware.org/images/7/74/Title-LOGO6.jpg);
} /* ====================
以下、特殊なclassに適用される ==================== */
#navigation { position:absolute;
top:120px; width:970px; height:92px; background-repeat: no-repeat;
color: #0000FF; }
/* ====================
ここからプルダウン周辺のデザイン ==================== */
- menu * {
margin: 0; padding: 0; }
- menu {
position:absolute;
font-family: calibri, verdana, sans-serif;
font-color: #000000;
background-color: transparent; float:left; display: block; }
- menu ul {
position:relative;
float: left; text-align:center; list-style: none; }
- menu li {
background-color:yellow;
position:relative;
float:left;
width: 136px;
font-size: 23px;
font-weight: bold;
border:ridge 0px #FFFFFF; }
- menu li.ach {
background-color:yellow;
position:relative;
float:left;
width: 162px;
font-size: 23px;
font-weight: bold;
border:ridge 0px #FFFFFF; }
- menu li.sup {
background-color:yellow;
position:relative;
float:left;
width: 109px;
font-size: 17px;
font-weight: bold;
border:ridge 0px #FFFFFF; }
- menu li.none {
background-color:yellow;
position:relative;
float:left;
width: 152px;
font-size: 17px;
font-weight: bold;
border:ridge 0px #FFFFFF; }
- menu a {
color: #000000; display:block; text-decoration: none; }
- menu a:hover {
background-color:gold; }
- column-one { display:none; width:0px;}
.container{background-color: #ffffff; margin-top:0px} .OWWNBcpCurrentDateFilled {display: none;}
- footer{position: center; width: 994px}
@media screen {
body { background: #F5F5F5 0 0 no-repeat; /* changed default background */ }
} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>
We have set 3 goals, that is "Energy production for rail-free movement", "Speeding up", and "Direction control". |
Methods
<html><A href=#TOP title="Vapor deposition"><IMG width="300px" src="http://openwetware.org/images/a/a5/Vapor_deposition.jpg"></A></html> <html><A href=#TOP title="SAM conjugation"><IMG width="300px" src="http://openwetware.org/images/4/48/Thiol_.jpg"></A></html> <html><A href=#TOP title="EDAC conjugation"><IMG width="300px" src="http://openwetware.org/images/3/3d/EDAC_conjugation.jpg"></A></html> <html><A href=#TOP title="DNA design"><IMG width="300px" src="http://openwetware.org/images/b/b2/DNA_design.jpg"></A></html> <html><A href=#TOP title="半白金 1μの動画"><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html> <html><A href=#TOP title=DNA hybrudization><IMG width="300px" src="http://openwetware.org/images/f/f1/DNA_hybrudization.jpg"></A></html>
<html><A href=#TOP title="ハイスピードカメラ"><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html> <html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/e/ed/%E3%82%AB%E3%82%BF%E3%83%A9%E3%83%BC%E3%82%BC.jpg"></A></html> <html><A href=#TOP title="Azobenzene"><IMG width="300px" src="http://openwetware.org/images/e/ea/Azobenzene.jpg"></A></html>
Vapor deposition of Au and Cr on the polystyrene body
File:Body construction.png Vapor deposition is a method for forming a thin metal film on the surface of the substrate. In this project, Gold and Chromium were deposited on the polystyrene beads in order to conjugate specific DNA onto the determined location on the surface.
After PVD
- Through twice of physical vapor deposition,
- we conjugate thiol modified DNA onto gold part.
- we conjugate amino modified DNA onto polystyrene part.
- There are no DNA staple strands on chromium hemisphere.
- Therefore, platinum particles can't conjugate onto chromium hemisphere.
- As a result, JET starts to move toward chromium hemisphere heading.
- Through twice of physical vapor deposition,
DNA conjugation
File:Body construction.png SAM We bond Au with DNA using thiol.
DNA Design
We used NUPACK and designed five strands of DNA.
- >>see more Method
Observation of platinum hemisphere
DNA hybridization in solution of H2O2
We used PAGE electrophoresis to ascertain the stability of DNA duplex in thin H₂O₂ solution 1%~5%.