Haynes Lab:Notebook/CRISPR Editing/2014/10/07: Difference between revisions
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Rene M Davis (talk | contribs) |
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Possibility 1: I made a mistake and added template to my no template controls <br> | Possibility 1: I made a mistake and added template to my no template controls <br> | ||
::Counter: The likely way I would do this would be to add a correctly diluted sample but the Cp of these wells is later than my lowest dilution on my curve | ::Counter: The likely way I would do this would be to add a correctly diluted sample but the Cp of these wells is later than my lowest dilution on my curve | ||
::Counter: Doesn't explain bands of different sizes for same primer set | |||
Possibility 2: I'm only measuring primer dimer <br> | Possibility 2: I'm only measuring primer dimer <br> | ||
::Counter: Why are my dilution curves so perfect? Seems the Cp is dependent on template concentration. | ::Counter: Why are my dilution curves so perfect? Seems the Cp is dependent on template concentration. |
Revision as of 21:18, 7 October 2014
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10/07/2014Ran a gel of some of the samples from the qPCR plate to check for primer dimer products
Possibility 2: I'm only measuring primer dimer
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