Haynes Lab:Notebook/CRISPR Editing/2014/12/17: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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==07//2014==
==12/17/2014==
 
Gradient PCR of g029 treated Luc14 gDNA. 6 temperatures, 50ul reactions.<br>
 
:53°C
:55°C
:57°C
:59°C
:61°C
:63°C
Ran gel, only saw additional bands at 53°C, didn't see any drop off in yield. <br>
Set up 50ul PCR reactions for g023, g025, g030, untreated luc14 gDNA. annealing temp 63.


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Latest revision as of 00:36, 27 September 2017

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12/17/2014

Gradient PCR of g029 treated Luc14 gDNA. 6 temperatures, 50ul reactions.

53°C
55°C
57°C
59°C
61°C
63°C

Ran gel, only saw additional bands at 53°C, didn't see any drop off in yield.
Set up 50ul PCR reactions for g023, g025, g030, untreated luc14 gDNA. annealing temp 63.


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