Haynes Lab:Notebook/CRISPR Editing/2015/01/23: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==01/23/2015==
==01/23/2015==
cutting 1μg of untreated, g033, and g034 treated Luc14 cells gDNA PCRd with P197/P198 and column purified samples from 14.12.06 experiment with PfoI at 37°C for 3 hours.
Sent out untreated Luc14 gDNA PCR product of P197/P198 for sequencing:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Label'''
| align="center" style="background:#f0f0f0;"|'''Primer'''
| align="center" style="background:#f0f0f0;"|'''Direction'''
|-
| RD-1-3||P175||F
|-
| RD-4-6||P161||F
|-
| RD-7-9||P173||F
|-
| RD-10-12||P159||F
|-
| RD-13-15||P170||R
|-
| RD-16-18||P156||R
|-
| RD-19-21||P172||R
|-
| RD-22-24||P158||R
|}
<br><br>
'''Quantification of editing efficiency with restriction enzyme''''<br>
 
cutting 1μg of untreated and g033 and g034 treated Luc14 cells gDNA PCRd with P197/P198 and column purified samples from 14.12.06 experiment with PfoI at 37°C for 3 hours.
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample Read#'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 1ug'''
| align="center" style="background:#f0f0f0;"|'''ul H2O'''
| align="center" style="background:#f0f0f0;"|'''ul buffer'''
| align="center" style="background:#f0f0f0;"|'''ul PfoI'''
|-
| Untreated||85.402||11.71||5.29||2||1
|-
| g033||78.942||12.67||4.33||2||1
|-
| g034||80.841||12.37||4.63||2||1
|}
 
'''Quantification with 1% agarose gel'''
 
 
<br><br>
 
 




Latest revision as of 00:40, 27 September 2017

Project name Main project page
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01/23/2015

Sent out untreated Luc14 gDNA PCR product of P197/P198 for sequencing:

Label Primer Direction
RD-1-3 P175 F
RD-4-6 P161 F
RD-7-9 P173 F
RD-10-12 P159 F
RD-13-15 P170 R
RD-16-18 P156 R
RD-19-21 P172 R
RD-22-24 P158 R



Quantification of editing efficiency with restriction enzyme'

cutting 1μg of untreated and g033 and g034 treated Luc14 cells gDNA PCRd with P197/P198 and column purified samples from 14.12.06 experiment with PfoI at 37°C for 3 hours.

Sample Read# ng/µL ul for 1ug ul H2O ul buffer ul PfoI
Untreated 85.402 11.71 5.29 2 1
g033 78.942 12.67 4.33 2 1
g034 80.841 12.37 4.63 2 1

Quantification with 1% agarose gel







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